In order to solve the inhibition problem on anaerobic ammonia oxidation (Anammox) process by new contaminants in the antibiotic class produced in chemical pharmaceutical industries, the inhibition kinetics was investigated by using chlortetracycline (CTC) as a representative and the alleviating efficiency of MIL101(Fe) on Anammox after prepared. The results showed that the addition of 30 mg/L CTC could significant inhibit Anammox process and total nitrogen removal was reduced by 19.43%. Both remodified Boltzmann model and modified Gompertz model could analyze the inhibition process of CTC. The addition of MIL100(Fe) could significantly reduce the free CTC concentration, significant promote the secretion of EPS, and increase the growth and metabolic activity of Anammox bacteria, thus resulting in high nitrogen removal. The results provide a theoretical and technical foundation for the application of Anammox in chemical and pharmaceutical wastewater treatment.