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| 胜利油田沾3区块油藏中Geobacillus菌的激活研究 |
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李彩风1,2,李阳3,吴昕宇4,曹嫣镔2,汪卫东2,包木太1
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(1.中国海洋大学化学化工学院,山东青岛 266100;2.中国石化股份胜利油田分公司采油工艺研究院,山东东营 257000;3.中国石油化工股份有限公司,北京 100728;4.中国石油大学信息与控制工程学院,山东青岛 266580)
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| 摘要: |
| 利用16S rRNA克隆文库技术分析胜利油田沾3区块油藏样品的微生物群落结构,使用不同激活剂对沾3区块油藏样品进行内源微生物激活,对激活后样品进行乳化能力、产生表面活性物质能力评价及微生物群落结构分析,并开展物理模拟驱油实验。结果表明:沾3区块油藏样品中含有2％的Geobacillus,该菌是沾3区块油藏内源微生物中产生表面活性物质、发挥乳化功能的关键菌群;加入适宜的激活剂体系可以选择性激活该类细菌,使其成为优势菌;利用选择性激活Geobacillus的配方激活沾3区块油藏内源微生物,可以在水驱基础上提高原油采收率10.8％。 |
| 关键词: Geobacillus 乳化能力 激活 内源微生物 微生物种群 |
| DOI：10.3969/j.issn.1673-5005.2016.01.023 |
| 分类号::TE 357.9 |
| 基金项目:国家“863”计划项目(2013AA064401) |
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| Study on the activation Geobacillus species in Zhan 3 blocks of Shengli Oilfield |
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LI Caifeng1,2, LI Yang3, WU Xinyu4, CAO Yanbin2,WANG Weidong2, BAO Mutai1
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(1.College of Chemistry ＆ Chemical Engineering in Ocean University of China, Qingdao 266100, China;2.Shengli Oil Production Research Institution, SINOPEC, Dongying 257000, China;3.China Petroleum ＆ Chemical Corporation, Beijing 100728, China;4.College of information and Control Engineering in China University of Petroleum, Qingdao 266580, China)
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| Abstract: |
| The microbial community structure in Zhan 3 block reservoir of Shengli Oilfield was analyzed by means of 16S rRNA clone library. The indigenous microorganism in Zhan 3 block reservoir was activated by adding different activators. And the emulsifying ability, the ability of producing surfactant, and the analysis of microbial community structure for the activated samples were studied. The physical simulation flooding experiments were also carried out. The results show that Geobacillus accounts for 2％ of the total sequences in Zhan 3 reservoir samples, which is the key species for producing surfactant and playing the function of emulsification. It could be selectively activated and becomes the dominant species by adding proper activators. The indigenous microorganism could enhance oil recovery up to 10.8％ based on water flooding at the Geobacillus activator formula in the reservoir conditions. |
| Key words: Geobacillus emulsifying ability activation indigenous microorganism microbial population |
