| 摘要: |
| 从油田采出水中富集培养石油烃降解菌,利用13C标记的甲苯作为碳源和变性梯度凝胶电泳技术,从中鉴定和考察能够降解甲苯的细菌群落。结果表明:从采出水中富集培养的复合菌群经过500 d的厌氧降解,对石油烃降解率达到36.4％,甲烷产量为201.2 μmol; 以13C标记的甲苯为碳源, 经过60 d 的培养,复合菌群对其降解率达到67％,12C标记的甲苯降解率为69％,两种碳源标记甲苯的降解率差异不显著；将离心中的重带13C-DNA片段回收测序,鉴别出3种芳烃降解菌。 |
| 关键词: 碳同位素 菌株鉴定 CsCl密度梯度 DGGE分析 芳烃 |
| DOI：10.3969/j.issn.1673-5005.2015.06.007 |
| 分类号::T 992.4 |
| 基金项目:中国石油天然气集团公司科学研究与技术开发项目( 2008D-4704-2) |
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| Identification of arene degradation bacteria using carbon isotope labeling method |
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HU Hengyu, ZHAO Dongfeng, LIU Chunshuang
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(College of Chemical Engineering in China University of Petroleum, Qingdao 266580, China)
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| Abstract: |
| The degradation bacteria of petroleum hydrocarbon were enriched and cultured from the oilfield water. Using 13C-toluene as carbon source and denaturing gradient gel electrophoresis (DGGE) technology to identify and investigate the bacterial community which could degrade the toluene. The results show that after 500 days anaerobic degradation the petroleum hydrocarbon degradation rate is 36.4％ by complex bacterium, and the methane yield is 201.2 μmol. It is also found that after 60 days culture the carbon source13C-toluene degradation rate is 67％ by the complex bacterium. Meanwhile, after 60 days culture the 12C-toluene degradation rate is 69％, and two kinds of toluene degradation rate are not significantly different. 13C-DNA is recovered and identified for three types of aromatic hydrocarbon degrading bacteria. |
| Key words: carbon isotope bacteria identification CsCl density gradient DGGE analysis arene |
